schisto research community

content appropriate for schistosomiosis research

13 Oct

Frustration with recrystallization

Published by RyanPakula

Subject 

Request for Help

Last Wednesday I began some simple tests to try to find a good solvent for recrystallization, and here's some details and thoughts on what I've found so far.

13 Oct

Recrystallization of PZQ

Published by RyanPakula

This information is from a few days previous, but I didn't have the blog all set up then, so here it is now.
 
As the first step in working with praziquantel, I'm looking to simply find some pretty solid conditions for recystallizing the racemate.  Ideally we'd be able to separate the enantiomers via recrystallization of a scalemic sample (which is already reported, vide infra), but later it'll be important that we can at least purify the racemate this way, as opposed to using more expensive methods such as chromatography.
 

08 Sep

Help for molecular analysis of S.haematobium from cameroon

Published by luogbou

I am Luogbou-nzu, PhD student at the university of Yaoundé I camerron. I am working on genetic diversity of Schistosoma haematobium from Cameroon.  At this level I have already collected samples from 10 villages in Cameroon.

02 Sep

Resistance to Praziquantel

Published by MatTodd

Subject 

External News

There is often talk of whether (or when) resistance to praziquantel will develop, and how catastrophic this would be for the treatment of schisto. There have been some important studies on this over the years, but a recent paper in PLoS NTD provides some new evidence for the development of resistance in Kenya. Thanks to Marc for the heads up.

31 Jan

Quick status update

Published by MatTodd

Subject 

Miscellaneous

A quick status update. While we were successful in getting our grant with the Australian Research Council and the World Health Organisation, we have so far been unable to formulate a contract that is mutually acceptable to all parties. Negotiations are continuing, but this explains the lack of recent activity coming from our lab on the schisto projects - we don't have the money yet! Fingers crossed this situation will resolve itself positively in the near future.

31 Jan

Misc articles

Published by MatTodd

Subject 

Miscellaneous

The Guardian Weekly's a lovely thing (particularly if you don't live in the UK). Two articles in yesterday's edition caught my eye.

Role of Calcium Flux

Published by MatTodd on 1 June 2008 - 9:31pm

Another interesting PZQ paper has come out of the cell biology group at the CNR in Rome, concerning the mode of action of PZQ. Using radioactive calcium, the group have demonstrated that calcium influx in itself is not enough to kill parasites. Pre-incubation with cytochalasin D promoted calcium influx but protected the worms from PZQ's schistosomicidal effects.

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