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A genetic screen in rodent malaria parasites identifies five new apicoplast putative membrane transporters, one of which is essential in human malaria parasites

CiteULike malaria tags - 20 September 2017 - 9:23am
Cellular Microbiology (13 September 2017), e12789, doi:10.1111/cmi.12789
Claire Sayers, Vanessa Mollard, Hayley Buchanan, Geoffrey McFadden, Christopher Goodman
Categories: malaria news feeds

PhenoPlasm: a database of disruption phenotypes for malaria parasite genes

CiteULike malaria tags - 4 August 2017 - 8:08am
Wellcome Open Research, Vol. 2 (21 June 2017), 45, doi:10.12688/wellcomeopenres.11896.1
Theo Sanderson, Julian Rayner
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An improved Plasmodium cynomolgi genome assembly reveals an unexpected methyltransferase gene expansion

CiteULike malaria tags - 3 August 2017 - 12:39pm
Wellcome Open Research, Vol. 2 (16 June 2017), 42, doi:10.12688/wellcomeopenres.11864.1
Erica Pasini, Ulrike Böhme, Gavin Rutledge, Annemarie Voorberg-Van der Wel, Mandy Sanders, Matt Berriman, Clemens Kocken, Thomas Otto
Categories: malaria news feeds

Rapid evolution of female-biased genes among four species of Anopheles malaria mosquitoes

CiteULike malaria tags - 3 August 2017 - 12:22pm
Genome Research (26 July 2017), gr.217216.116, doi:10.1101/gr.217216.116

An international, peer-reviewed genome sciences journal featuring outstanding original research that offers novel insights into the biology of all organisms
Francesco Papa, Nikolai Windbichler, Robert Waterhouse, Alessia Cagnetti, Rocco D'Amato, Tania Persampieri, Mara Lawniczak, Tony Nolan, Philippos Papathanos
Categories: malaria news feeds

The exported chaperone Hsp70-x supports virulence functions for Plasmodium falciparum blood stage parasites

CiteULike malaria tags - 27 July 2017 - 10:09am
PLOS ONE, Vol. 12, No. 7. (21 July 2017), e0181656, doi:10.1371/journal.pone.0181656

Malaria is caused by five different Plasmodium spp. in humans each of which modifies the host erythrocyte to survive and replicate. The two main causes of malaria, P. falciparum and P. vivax, differ in their ability to cause severe disease, mainly due to differences in the cytoadhesion of infected erythrocytes (IE) in the microvasculature. Cytoadhesion of P. falciparum in the brain leads to a large number of deaths each year and is a consequence of exported parasite proteins, some of which modify the erythrocyte cytoskeleton while others such as PfEMP1 project onto the erythrocyte surface where they bind to endothelial cells. Here we investigate the effects of knocking out an exported Hsp70-type chaperone termed Hsp70-x that is present in P. falciparum but not P. vivax. Although the growth of Δhsp70-x parasites was unaffected, the export of PfEMP1 cytoadherence proteins was delayed and Δhsp70-x IE had reduced adhesion. The Δhsp70-x IE were also more rigid than wild-type controls indicating changes in the way the parasites modified their host erythrocyte. To investigate the cause of this, transcriptional and translational changes in exported and chaperone proteins were monitored and some changes were observed. We propose that PfHsp70-x is not essential for survival in vitro, but may be required for the efficient export and functioning of some P. falciparum exported proteins.
Sarah Charnaud, Matthew Dixon, Catherine Nie, Lia Chappell, Paul Sanders, Thomas Nebl, Eric Hanssen, Matthew Berriman, Jo-Anne Chan, Adam Blanch, James Beeson, Julian Rayner, Jude Przyborski, Leann Tilley, Brendan Crabb, Paul Gilson
Categories: malaria news feeds

Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes

CiteULike malaria tags - 20 July 2017 - 12:15pm
Cell, Vol. 170, No. 2. (July 2017), pp. 260-272.e8, doi:10.1016/j.cell.2017.06.030
Ellen Bushell, Ana Gomes, Theo Sanderson, Burcu Anar, Gareth Girling, Colin Herd, Tom Metcalf, Katarzyna Modrzynska, Frank Schwach, Rowena Martin, Michael Mather, Geoffrey McFadden, Leopold Parts, Gavin Rutledge, Akhil Vaidya, Kai Wengelnik, Julian Rayner, Oliver Billker
Categories: malaria news feeds

Rapid identification of genes controlling virulence and immunity in malaria parasites

CiteULike malaria tags - 20 July 2017 - 11:54am
PLOS Pathogens, Vol. 13, No. 7. (12 July 2017), e1006447, doi:10.1371/journal.ppat.1006447

Identifying the genetic determinants of phenotypes that impact disease severity is of fundamental importance for the design of new interventions against malaria. Here we present a rapid genome-wide approach capable of identifying multiple genetic drivers of medically relevant phenotypes within malaria parasites via a single experiment at single gene or allele resolution. In a proof of principle study, we found that a previously undescribed single nucleotide polymorphism in the binding domain of the erythrocyte binding like protein (EBL) conferred a dramatic change in red blood cell invasion in mutant rodent malaria parasites Plasmodium yoelii. In the same experiment, we implicated merozoite surface protein 1 (MSP1) and other polymorphic proteins, as the major targets of strain-specific immunity. Using allelic replacement, we provide functional validation of the substitution in the EBL gene controlling the growth rate in the blood stages of the parasites. Developing a greater understanding of malaria genetics is a key step in combating the threat posed by the disease. Here we use a novel approach to study two important properties of the parasite; the rate at which parasites grow within a single host, and the means by which parasites are affected by the host immune system. Two malaria strains with different biological properties were crossed in mosquitoes to produce a hybrid population, which was then grown in naïve and vaccinated mice. Parasites with genes conveying increased growth or immune evasion are favoured under natural selection, leaving a signature on the genetic composition of the cross population. We describe a novel mathematical approach to interpret this signature, identifying selected genes within the parasite population. We discover new genetic variants conveying increased within-host growth and resistance to host immunity in a mouse malaria strain. Experimental validation highlights the ability of this rapid experimental process for generating insights into malaria biology.
Hussein Abkallo, Axel Martinelli, Megumi Inoue, Abhinay Ramaprasad, Phonepadith Xangsayarath, Jesse Gitaka, Jianxia Tang, Kazuhide Yahata, Augustin Zoungrana, Hayato Mitaka, Arita Acharjee, Partha Datta, Paul Hunt, Richard Carter, Osamu Kaneko, Ville Mustonen, Christopher Illingworth, Arnab Pain, Richard Culleton
Categories: malaria news feeds

Common Piezo1 allele in African populations causes xerocytosis and attenuates Plasmodium infection

CiteULike malaria tags - 7 July 2017 - 10:00am
bioRxiv (06 July 2017), 159830, doi:10.1101/159830

bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
Shang Ma, Stuart Cahalan, Rakhee Lohia, Gregory LaMonte, Weizheng Zeng, Swetha Murthy, Emma Paytas, Nathan Grubaugh, Ramya Gamini, Laurence Berry, Viktor Lukacs, Tess Whitwam, Meaghan Loud, Andrew Su, Kristian Andersen, Elizabeth Winzeler, Eric Honore, Kai Wengelnik, Ardem Patapoutian
Categories: malaria news feeds

Nutrient sensing modulates malaria parasite virulence

CiteULike malaria tags - 6 July 2017 - 10:50am
Nature, Vol. advance online publication (05 July 2017), doi:10.1038/nature23009
Liliana Mancio-Silva, Ksenija Slavic, Margarida Grilo Ruivo, Ana Grosso, Katarzyna Modrzynska, Iset Vera, Joana Sales-Dias, Ana Gomes, Cameron MacPherson, Pierre Crozet, Mattia Adamo, Elena Baena-Gonzalez, Rita Tewari, Manuel Llinás, Oliver Billker, Maria Mota
Categories: malaria news feeds

Nutrient sensing modulates malaria parasite virulence

CiteULike malaria tags - 6 July 2017 - 4:59am
Nature, Vol. advance online publication (05 July 2017), doi:10.1038/nature23009
Liliana Mancio-Silva, Ksenija Slavic, Margarida Grilo Ruivo, Ana Grosso, Katarzyna Modrzynska, Iset Vera, Joana Sales-Dias, Ana Gomes, Cameron MacPherson, Pierre Crozet, Mattia Adamo, Elena Baena-Gonzalez, Rita Tewari, Manuel Llinás, Oliver Billker, Maria Mota
Categories: malaria news feeds

Panoptes: web-based exploration of large scale genome variation data

CiteULike malaria tags - 30 June 2017 - 9:59am
Bioinformatics (23 June 2017), doi:10.1093/bioinformatics/btx410
Paul Vauterin, Ben Jeffery, Alistair Miles, Roberto Amato, Lee Hart, Ian Wright, Dominic Kwiatkowski
Categories: malaria news feeds

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