Tuberculosis Research Community

Welcome, I'm Miguel Mitchell, the online leader for The Synaptic Leap tuberculosis research community.

Why Tuberculosis Is Important

Tuberculosis (TB), the disease caused by Mycobacterium tuberculosis (Mtb), infects approximately two billion people. The World Health Organization estimates that about two million people die each year from TB due to the lack of and inability to afford proper health care. Overcrowding and ill-nourishment of poor people living in large cities leads to ahigh incidence of the disease due to the ease at which the infection can be transferred. This contributes to the accelerated speed at which TB spreads in underdeveloped countries. There is also an alarming increase in cases of TB caused by multidrug-resistant strains of Mycobacterium tuberculosis (Mtb), due in part to inadequate drug therapy as a result of incorrectly selected medications or suboptimal drug dosing. Thus, there is a need for new drugs targeting enzymes essential to mycobacterial survival. 

Join Us and Login

As a guest on The Synaptic Leap, you can browse and look all you want.  You must login and create a user profile for yourself if you want to participate. Once you are logged in you may begin collaborating with other scientists world-wide and become a part of the collective intelligence trying to help improve the drugs available for schisto. 

Get Involved with an Open Research Project 

See current projects for a list of our active open research projects.  You may learn more about these projects and learn how you can participate.  Or, you may "add a child page" (see the link at the bottom) to initiate and describe your own open research project for tuberculosis.

If you're still in the brainstorming phase of starting a project, write a blog article to discuss your ideas with other scientists around the world also studying TB. Working together, we can direct the research towards the most promising ideas.

You can also help shape and direct other TB research ideas by reading and commenting on other community posts

Keep Up To Date with The Latest TB Information

To assist you with your tuberculosis research we have pulled together a research tools page and an RSS news feeds for TB.  If you know of a useful tool that we don't have on the list, login and add a comment for others to see.  You can also push articles to our RSS news feeds by using the "Tuberculosis" tag on either Connotea or CiteULike.

 

Current Projects for Tuberculosis

All tuberculosis projects currently in process should be created as child pages to this page.  See the "add child" link at the bottom if you want to initiate a new open research project for TB.

Development of Novel Antitubercular Drugs

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This is a call for creating a tuberculosis research community.

Background

Tuberculosis (TB), the disease caused by Mycobacterium tuberculosis (Mtb), infects approximately two billion people. The World Health Organization estimates that about two million people die each year from TB due to the lack of and inability to afford proper health care.1 Overcrowding and ill-nourishment of poor people living in large cities leads to a high incidence of the disease due to the ease at which the infection can be transferred.2 This contributes to the accelerated speed at which TB spreads in underdeveloped countries. There is also an alarming increase in cases of TB caused by multidrug-resistant strains of Mycobacterium tuberculosis (Mtb), due in part to inadequate drug therapy as a result of incorrectly selected medications or suboptimal drug dosing.3 Thus, there is a need for new drugs targeting enzymes essential to mycobacterial survival.

Research Focus 

My lab is focusing on the inhibition of type II NADH-menaquinone dehydrogenase (ndh-2). By inhibiting ndh-2, the electron transport chain in Mtb becomes blocked and shuts down. Ndh-2 is the only NADH dehydrogenase enzyme expressed in Mtb and is thus vital to its survival.4 Ndh-2 is also found in a number of other bacteria such as Staphylococcus aureus and Enterococcus faecalis but is not expressed in humans.5 Humans rely only on type I NADH dehydrogenase (ndh-1) and thus minimal toxicity in humans is predicted with ndh-2 inhibitors.

Current State of Affairs

My lab has discovered two quaternized promazine derivatives (QPDs), 1 and 2, that both have an MIC (minimum inhbitory concentration) of 3.13 μg/mL vs. Mycobacterium tuberculosis H37Rv and SI values (IC50 vs. human Vero cells/MIC) of 7.6 and 4.1, respectively. 

 compounds 1 and 2

Changing the para substituent to H, CH3O, or NO2 (or replacing benzyl by allyl) raises the MIC.  The pharmacophore definitely needs something aromatic on the quaternized N (84% inhibition for benzyl at 6.25 μg/mL; only 47% inhibition for allyl at 6.25 μg/mL). Our goal:  MIC ≤ 3.13 μg/mL against both current drug regimen-sensitive and multidrug-resistant Mtb and SI ≥ 30.

Although my lab can do good synthetic work, we are solely a post-doc free, undergraduate chemistry department and need assistance in several areas:

  • Antitubercular testing.  So far we have depended on TAACF (Tuberculosis Antimicrobial Acquisition and Coordinating Facility) to get free MIC and SI data.  TAACF is a great resource, but it takes over a year to get data!!  We want to develop better TB cures in a reasonable (<10 yr) timeline. We'd like to work with a group or groups dedicated to testing our compounds and willing to post the MIC and SI data on this site to speed up critical evaluation of potential leads. 
  • Proposing potential ndh-2 inhibiting antitubercular compounds.
  • Letting us know what doesn't work (too toxic, high MIC).
  • Helping with inexpensive synthetic routes to targets.  TB is predominately a disease of the poor and we must be able to scale up the chemistry at low cost.
  • Helping with greener synthetic routes to targets.  It would be great to have efficient atom-economy and minimum hazardous waste production.
  • Synthesizing new QPDs or analogs.  The goal is to stop TB, so go for it!  Just let us know what's happening on this site so that we (and others) don't reinvent the wheel.

This is my first time freely discussing yet to be published medicinal chem research.  It is both daunting and invigorating.  I hope that there are some allies in this effort to stop TB.

1. Maher, D. & Raviglionem, M. C. (1999) in Tuberculosis and Nontuberculous
Mycobacterial Infections, ed. Schlossberg, D. (Saunders, Philadelphia), 4th Ed., pp.104-115.
2. Lowell, A. M. (1999) in Tuberculosis and Nontuberculous Mycobacterial Infections, ed. Schlossberg, D. (Saunders, Philidelphia), 4th Ed., pp. 3-15.
3. Bearing, S. E.; Peloquin, C.A.; Patel, K.B. (1999) in Tuberculosis and Nontuberculous Mycobacterial Infections, ed. Schlossberg, D. (Saunders,
Philadelphia), 4th Ed., pp. 83-91.
4. Weinstein, E.A.; Yano, T.; Li, L.S.; Avarbock, D.; Avarbock, A.; Helm, D.; McColm, A.A.; Duncan, K.; Lonsdale, J.T.; Rubin, H. PNAS 2005, 102, 4548-4553.
5. Melo, A. M. P.; Bandeiras, T. M.; Teixeira, M. Microbiol. Mol. Biol. Rev. 2004, 68, 603-616. 

Development of Polypharmacologic Ser/Thr kinase Inhibitors

Subject 

Request for Help

Background

The idea is to design Potential Kinase inhibitors using Pharmacophore search, Docking, Semantic link association prediction, VROCS and fragment based approach to find novel molecules against Kinases.PASS Prediction

Description

The project is divided into several sections Each section would result in different compounds. The final docking is done in Moldock and the compounds are ranked according to the rerank scores .After ranking compounds by several methods final ranking would be done using data fusion methods. ADMET filters would be applied to check whether the compounds are feasible to test in wet labs.

Challenges

Identification of Pharmacophore , fragment based approach, docking and scoring. 

Idea Details

I did use the some Ligand based Techniques to identify Novel Inhibitors of PkNb and this will get extended further for other kinase Inhbitors. Finding the Ligand binding pattern of Kinase inhibitors.

 
Identification of Mycobacterium H37Rv Pknb inhibitors.pdf